Hormonal dynamics in the preovulatory ovarian follicle and the oocyte in rat during proestrus
نویسنده
چکیده
We studied functional and morphological luteinization of granulosa cells in intact preovulatory follicles and in cultured granulosa cells collected from the proestrous follicles of the rat. Steroid hormones were measured by radioimmunoassay in follicles dissected from the ovaries every 2 hrs on the day of proestrus beginning at 8 : 00 hrs and terminating at the time of ovulation. Granulosa cells for culture were collected in the same intervals. We simultaneously observed the morphology of granulosa cells and follicle-enclosed oocytes in vivo.
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